Abstract Detail

Anatomy and Morphology

Spiegeler, McKayla [1], Klahs, Phillip [1], Clark, Lynn [2].

The isolation of lobed chlorenchyma cells from grass (Poaceae) leaves using a soft tissue maceration technique.

The complexity of cell form in the chlorenchyma of grasses is easily missed with conventional cross sectional light microscopy. A recent survey of chlorenchyma cell shapes within the family Poaceae using 3-dimensional reconstruction of images from longitudinal, paradermal, and cross sections exposed a range of shape modification with various degrees of cellular lobing among the taxa studied. The significance of the specialized chlorenchyma cells is not fully understood and attempting to quantify their shape, while the cells remain compacted in the mesophyll, often proves difficult. In order to verify previous measurements and further the research on these cells a maceration technique was refined to isolate mesophyll cells from grass leaves. Leaves of Triticum aestivum (wheat), which have highly lobed mesophyll cells designated as “radiator” cells, were removed from plants growing in a greenhouse and then fixed in gFAA. The fixed plant material was transferred through a series of ethanol solutions starting at 50% ethanol and ending in distilled water, then placed on a microscope slide, and covered in an aqueous pectinase solution. Careful dissection with a razor blade was used to mechanically separate mesophyll from vascular bundles and epidermis. The pectinase solution dissolves the middle lamella and with additional agitation the mesophyll breaks into individual cells. The isolated cells may be subjected to biological stains and dyes to improve imaging or explore cellular composition.

1 - Iowa State University, Ecology, Evolutionary, and Organismal Biology, 251 Bessey Hall, 2200 Osborn Drive, Ames, Iowa, 50011, United States
2 - Iowa State University, Department Of Ecology, Evolution, And Organismal Biology, 251 Bessey Hall, 2200 Osborn Dr., Ames, IA, 50011, United States


Presentation Type: Poster
Number: PAM001
Abstract ID:95
Candidate for Awards:Developmental and Structural Section best poster

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