Abstract Detail



Symbioses: Plant, Animal, and Microbe Interactions

Meyer, Rachel [1], DeVries, Amber [2], Wright, Jessica [3], Curd, Emily [4], Sork, Victoria [5], Wayne, Robert [6].

The Valley Oak Leaf and Bulk Soil ‘Parabiome’ are Partially Shaped by Host Genetics.

Community networks in soil and on trees can influence plant traits, many having fitness consequences such as pathogens. Microorganisms influence herbivory, pathogen defense, stomatal regulation, water uptake, and the presence of microbial organisms such as arthropods. Likewise, arthropod communities on plants mediate microbial communities to gain access to plant habitats, such as leaf space, flowers, stems, roots and soil. Fungal communities have been shown to exhibit patterning on plant species with correlation to biogeography. Little work has been done to systematically characterize communities on and around plants, but the implications of communities on plant fitness suggest the need incorporate networks of nearby species, usually called, microbiomes, holobionts, phytobiomes, or here, parabiomes, into theory and models of  species and community evolution.  We performed an environmental DNA (eDNA) analysis of oak tree and oak garden parabiomes using two common gardens for Valley Oak (Quercus lobata) planted in 2015 with saplings. We sampled soil from under and around oak trees that were sourced as acorns from three different regions of California.  We also sampled leaves from these trees. Nine plants per common garden were used in the study.  DNA was extracted from the soil, from the phyllosphere, and the leaf endosphere. eDNA metabarcoding was performed with the 16S V4 region to target bacteria and archaea, the ITS1 region to target fungi, and the CO1 locus to target fungi and various invertebrates.   Results showed oaks decreased the richness of bacterial diversity in their surrounding soil, and exhibited a provenance drive of fungal and arthropod biodiversity in and on the leaves. This was robust to detect across the two gardens that had considerable parabiome differences. Beta diversity differences across kingdoms and families shows the clades that have site or provenance relationships. We also show which clades aren't tractable by metabarcoding. Results show the usefulness of eDNA to address research needs of managers, conservation practitioners, and molecular ecologists interested in taking on an eDNA project, and also reveals that oak genetics shape their local environmental communities.


Related Links:
https://sorklab.eeb.ucla.edu/
http://www.ucedna.com
ucconservationgenomics.eeb.ucla.edu


1 - UCLA, EEB, 610 Charles E Young Drive E, Room 4153, Los Angeles, CA, 90095, United States
2 - UCLA, 610 Charles E Young Dr. E, Los Angeles, CA, 90095, United States
3 - U.S.D.A. Forest Service, Pacific Southwest Research Station, 1731 Research Park Dr., Davis, CA, 95618, USA
4 - University of California Los Angeles, 610 Charles E Young Dr., TSLB 4153, Los Angeles, CA, 90095, United States
5 - UCLA, ECOL & EVOL BIOL, Box 957239 , Los Angeles, CA, 90095, United States
6 - University of California Los Angeles, EEB, 610 Charles E Young Dr., Los Angeles, CA, 90095, United States

Keywords:
Metagenomics
community ecology
eDNA
biodiversity
Microbiome
arthropods
endosymbionts
California
trees.

Presentation Type: Oral Paper
Number:
Abstract ID:869
Candidate for Awards:None


Copyright © 2000-2018, Botanical Society of America. All rights reserved