Abstract Detail



Comparative Genomics/Transcriptomics

Garza, Elyssa [1], Hawkins, Angela [2], Pepper, Alan [3].

Exploring serpentine tolerance in Caulanthus amplexicaulis through the integration of multiple genomic datasets.

A wild relative of Arabidopsis, Caulanthus amplexicaulis var. barbarae (Cab) is endemic to harsh serpentine soils; characterized by low calcium, toxic heavy metals such as nickel, limiting nutrients (e.g. N, P, K), and low water retention. Its sister variety, Caulanthus amplexicaulis var. amplexicaulis (Caa), inhabits adequate-nutrient granite soils. The distinct contrast between the natural habitats of these two plant varieties allows exploration into plant selective adaptations to serpentine. We have assembled, analyzed, and annotated the Cab and Caa genomes. The estimated genome size for C. amplexicaulis is 372 Mb, with 14 chromosomes. We compared whole-genome resequencing data, from a large set of recombinant inbred lines, against Cab and Caa parents to obtain a high-resolution linkage map of the Caulanthus genome. These data were integrated with phenotypic information to identify genomic intervals that underlie QTL for tolerance to low-calcium and toxic levels of Ni. Genome sequence scaffolds were anchored to the linkage map to provide a set of 14 annotated pseudo-chromosomes. Combining this genomic information with RNA-Seq expression data and other datasets will allow the identification of the specific genes underlying tolerance to low-calcium and Ni.


1 - Texas A&M University , 100 Butler hall, 3258 TAMU, College Station, TX, 77843, US
2 - Texas A&M University, Department Of Biology, 3258 TAMU, College Station, TX, 77843, United States
3 - Texas A&M University, Department Of Biology, Texas A&M University, TAMU 3258, 214 Bsbe, College Station, TX, 77843, United States

Keywords:
genome
Brassicaceae
Serpentine
Caulanthus
Adaptation.

Presentation Type: Oral Paper
Number:
Abstract ID:686
Candidate for Awards:Margaret Menzel Award


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