Abstract Detail



Population Genetics/Genomics

Umali, Johann [1], Obae, Samuel [2].

Phytochemical and genetic analysis of American mayapple.

American mayapple (Podophyllum peltatum) and Himalayan mayapple (P. hexandrum) are the two species of the genus Podophyllum that are commercially used as sources of podophyllotoxin, a bioactive constituent used in semi-synthesis of anti-cancer drugs such as Etoposide, Etopophos, and Teniposide. Due to the growing demand for podophyllotoxin, wild populations of P. hexandrum have been overharvested and the need for P. peltatum in on the rise. In this study we sought to quantify the podophyllotoxin content in leaves and rhizomes of P. peltatum using High Performance Liquid Chromatography (HPLC) and to determine the genetic diversity of its wild populations using UniGene-derived microsatellite markers of P. hexandrum. Our findings showed that podophyllotoxin content was significantly higher (P < 0.05) in leaves compared to rhizomes and roots. The correlation of podophyllotoxin content between above-ground (leaves) and below-ground (rhizomes and roots) tissues was positive but very weak (R2 = 0.03). Since leaves are renewable tissues and contain high podophyllotoxin content, they present a great potential to cultivate and commercialize P. peltatum as a sustainable crop to meet the growing demand for podophyllotoxin. Genetic analysis showed limited genetic variation within and among the two populations evaluated. This would be attributed to the clonal nature of this species and/or small sample size used in this study.


1 - Stevenson University, Biology, 11200 Ted Herget Way, Owings Mills, MD, 21117, USA
2 - Stevenson University, Biology, 11200 Ted Herget Way, Owings Mills, MD, 21117, United States

Keywords:
Podophyllotoxin
HPLC
SSR
Mandrake.

Presentation Type: Poster
Number: PPG005
Abstract ID:579
Candidate for Awards:None


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