Abstract Detail

Physiology & Ecophysiology

Wang, Xiaoyin [1], Yang, Mei [2], Downing, Jason [3], Liu, Hong [4], Manage Goodale, Uromi [5].

Temperature effects on the asymbiotic germination of two Paphiopelilum species.

Paphiopedilum, commonly known as the “lady’s slipper orchid” is a fascinating genus of orchids. The wild populations are under the threat of extinction as a result of over-collection and habitat loss, they are becoming increasingly rare due to the destruction of native habitats and illegal collection. All the wild paphiopedilum species are listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Ex situ conservation is a most effective way to protect the germplasm resources of Paphiopedilum. Asymbiotic germination provides a useful way to re-establish plants in the wild (conservation) and for commercial propagation so as to reduce pressure from illegal collection.     Here we examined the effects of temperature (low elevation and high elevation) on the asymbiotic germination rates on two Paphiopedilum species (P. dianthum and P. hirrsutissimum) and subsequent seed and protocorm development,observed the process of embryo development by microscope with the method of asymbiotic seed germination. Under the conditions of 23 and 27 degrees, the seeds were germinated and cultivated, and three important indexes of germination potential, germination rate and germination index were mainly studied. The results indicated that the lower temperature was better for the germination. And the highest rate of seed germination was obtained in around 200 days after seed sowing. Moreover, the embryo enlarged in 30 days and began to take an ellipsoidal shape.

1 - Guangxi University, Forestry College, 100, Daxuedonglu, Nanning, GX, 530004, China
2 - Guangxi Yachang Orchid National Nature Reserve Administration, Scientific Research Section, 46, Jinanlu, Nanning, GX, 530011, China
3 - Fairchild Tropical Botanic Garden, 10901,Old Cutler Rd, Miami, Florida, 33156, USA
4 - Florida International University, Department of Earth and Environment, 11200 SW 8th Street, Miami, Florida, 33199, USA
5 - Guangxi University, Guangxi Key Laboratory of Forest Ecology and Conservation, Forestry College, 100, Daxuedonglu, Nanning, GX, 530004, China

Seed Germination

Presentation Type: Oral Paper
Abstract ID:366
Candidate for Awards:Physiological Section Physiological Section Li-COR Prize,Physiological Section Best Paper Presentation

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