Abstract Detail

Comparative Genomics/Transcriptomics

Kim, Seon-Hee [1], Maki, Masayuki [2], Lepschi, Brendan  [3], Kim, Seung-Chul [1].

Comparative phylogenomic and structural analyses of complete plastomes of the most basal lineage Proto-Araceae.

The early diverging monocot family Araceae comprises over c. 3,800 species in 118 genera. The greatest diversity of these species occurs in the New World tropics, and exhibits diverse morphologies, high species diversity, a high endemism rate, and a wide range of basic chromosome numbers. This great diversity is also reflected in the wide variety of habitats occupied, mainly terrestrial and some aquatic herbs, vines, and epiphytes. The Proto-Araceae, which represents the earliest diverging in the family, includes two subfamilies, Gymnostachydoideae and Orontioideae. The subfamily Gymnostachydoideae includes one species in the Southern Hemisphere (Australia). The Northern Hemisphere subfamily Orontioideae includes three genera, i.e., Orontium (1 sp.), Lysichiton (2 spp.), and Symplocarpus (5 spp.). The genera Symplocarpus and Lysichiton are the best-known examples representing the eastern Asia, eastern North America/western North America disjunct distributions in the Northern Hemisphere. Despite their biogeographical and morphological features, and basal position within the family, no comparative plastome phylogenomics analysis of the Proto-Araceae has been completed. Therefore, we newly sequenced the plastome sequences of nine representative members of the Proto-Araceae [i.e., Gymnostachys anceps, Orontium aquaticum, Lysichiton camtschatcensis, L. americanus and three species of Symplocarpus (S. nipponicus, S. foetidus, and S. renifolius)]. Although there is a remarkably long evolutionary history of the Proto-Araceae, comparative genomic analyses have demonstrated that Proto-Araceae were highly conserved in terms of genomic length (156,868–163,063 bp), GC contents (37.3–37.7%), and gene organization and order. However, a few exceptions, which may indicate evolutionary events, found with the Proto-Araceae are as follows: (1) The ndhF gene inversion was found in two monotypic genera, Gymnostachys and Orontium, (2) The Gymnostachys and Orontium share the same 86 protein-coding genes, whereas all but one species of the Orontioideae have 85 protein-coding genes because the loss of the ycf1 gene, and (3) Among these lineages, the genera Gymnostachys and Orontium have the expanded range of inverted repeats ranging from 31.0 to 31.3 kb, whereas genera Lysichiton and Symplocarpus exhibited significant reductions in inverted repeats ranging from 24.0 to 25.8 kb. A phylogenetic analysis showed that the generic relationships within each group were all resolved. The phylogenetic relationships, plastome size and features for each species, SSR motifs, and highly variable plastome regions will be presented in detail.

1 - Sungkyunkwan University, Biological Sciences, 2066 Seobu-ro, Jangan-Gu, Suwon, 16419, Korea
2 - Tohoku University, Botanical Gardens, Kawauchi 12-2, Aoba, Sendai, Miyagi, 980-0862, Japan
3 - Australian National Herbarium, Centre for Australian National Biodiversity Research, Canberra, ACT, 2601, Australia
4 - Sungkyunkwan University, Biological Sciences, 2066 Seobu-ro, Jangan-Gu, Suwon, 16419, Korea

Comparative phylogenomics
Intercontinental disjunct distribution

Presentation Type: Poster This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Abstract ID:690
Candidate for Awards:None

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