Abstract Detail

Molecular Ecology

Messick, Jennifer [1].

Identification of Potential Gypsum Tolerance Candidate Loci using ddRADseq of Nama stevensii (Boraginaceae) and Phacelia integrifolia (Hydrophyllaceae).

A fundamental question in plant ecology is why are some plant species found only in certain habitat types and not others. This question leads to more specific questions when considering extreme habitats such as gypsum-based soil. Gypsum-based soils contain high levels of calcium sulfate dihydrate and are considered to be an extreme habitat for plants typically resulting in restricted growth and are considered to be of no potential use as agricultural soils. Even though this is an extreme habitat, some plant species only occur in this soil type and not in other soil types, two such species are Nama stevensii (Boraginaceae) and Phacelia integrifolia (Hydrophyllaceae).To investigate the potential molecular adaptation that allows for gypsum soil tolerance, leaf material from native populations of N. stevensii and P. integrifolia were sampled from 100 individuals of each species from gypsum outcrops in northwestern Oklahoma at the University of Central Oklahoma’s Selman Living Laboratory (approximately nine miles southwest of Freedom, OK; 36.712501, -99.258084). Nuclear DNA was extracted using the Qiagen Mini Plant Extraction Kit. ddRADseq library preparation and paired-end sequencing on an Illumina MiSeq were conducted at the University of Central Oklahoma, Edmond, OK. Each species was sequenced in a separate lane; 83 Nama stevensii individuals and 92 Phacelia integrifolia individuals. The Stacks pipeline was used to demultiplex pooled sequence data for each species and to calculate basic population statistics. Further analyses to identify outlier loci as candidates for the gypsum adaptation using BayeScan was unsuccessful in detecting outliers for each species individually. However, Stacks identified ten loci with eight variant sites shared between N. stevensii and P. integrifolia. Of these ten shared loci, seven were found to be significantly out of HWE for N. stevensii and eight for P. integrifolia. Pairwise FST values of the eight variant sites showed one with FST = 0.00, six with FST = 1.00, and one with FST = 0.97.These eight variant sites were determined to be the candidates with the highest probability of being associated with the gypsum tolerance trait of Nama stevensii and Phacelia integrifolia.

1 - University of Central Oklahoma, 100 N University Dr # 89, Edmond, Oklahoma, 73012, United States

none specified

Presentation Type: Oral Paper
Number: ME2006
Abstract ID:647
Candidate for Awards:None

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