Abstract Detail

Ferns at the extreme: the case of moonworts, grape-ferns and adder’s tongues of the family Ophioglossaceae

Koubínová, Darina [1], Kuo, Li-Yaung [2], Grant, Jason [1].

Infra-family relationships within Ophioglossaceae resolved with chloroplast and mitochondrial phylogenomics.

The Ophioglossaceae is a fern family composed of four ancient-diverged lineages, two of which are monotypic (subfamilies Helminthostachyoideae and Mankyuoideae). The relationships among these four subfamilies and the genera within them remain unresolved. Prior attempts to infer phylogenetic relationships, unfortunately, included limited plastid regions, and some even had a great portion of missing data. In two phylogenomic analyses, a plastome dataset was used but few representatives were sampled. Also, a rather simplified substitution model applied in these phylogenomic datasets may have introduced errors. To tackle the deepest and difficult nodes, we adopted a phylogenomic approach by adding more representatives (9 out of 12 currently recognized genera) and analyzed datasets from both the plastome and mitogenome. We used genome skimming data to assemble these organelle genomes and from the resulting assemblies, we extracted the coding sequences (CDS) for phylogenomic inference. We tested different partition and substitution models for these datasets, including finer ones to better account for rate heterogeneity among loci and codon positions. Our phylogenomic results overall support a novel, previously uncovered topology that shows the most solid backbone for Ophioglossaceae. Finally, based on this infra-family backbone, we traced phylogenetic origins of the hypothesized horizontal gene transfer (HGT) in organellar genomes, ancient whole genome duplication (WGD) events, and key morphological innovations in Ophioglossaceae.

1 - University of Neuchatel, Laboratory of evolutionary genetics, Emile-Argand 11, Neuchatel, Neuchatel, 2000, Switzerland
2 - National Tsing Hua University, Institute Of Molecular & Cellular Biology, Room 327, 3F, Life Science Building One, No.101, Sec., East, Hsinchu, HSZ, 30013, Taiwan
3 - University of Neuchatel, Laboratory of evolutionary genetics, Emile-Argand 11, Neuchatel, Neuchatel, 2000, Switzerland

genome skimming
coding sequence.

Presentation Type: Colloquium Presentations
Number: C4009
Abstract ID:579
Candidate for Awards:None

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