Abstract Detail

Ferns at the extreme: the case of moonworts, grape-ferns and adder’s tongues of the family Ophioglossaceae

Grant, Jason [1], Koubínová, Darina [1].

Mining into "extreme depths" of the Ophioglossaceae: A study of microsatellate content in Ophioglossaceae genomes.

The fern family Ophioglossaceae that comprises the moonworts (Botrychium), grape-ferns (Sceptridium), and adder’s tongues (Ophioglossum) is an ancient lineage with various strategies in reproduction, frequent hybridization, extremely high chromosome number in some groups, and unique symbioses with fungi. The family is found worldwide and has both temperate and tropical radiations. Species occur in primary forests to grasslands to disturbed areas. They may be terrestrial or epiphytic, common and widespread, or locally endemic. However, the simplified and variable morphology of most species causes species identification and circumscription challenging. There are over 100 species organized within about a dozen genera, some of which are speciose, and several that are monotypic. Most taxa remain poorly understood in terms of systematics or ecology. This symposium aims to report on recent advances in phylogenetics, taxonomy, systematics, regional floristics, and transcriptome analysis from a panel of local and international experts.In this first contribution, we discuss advances on studies on microsatellites, or simple sequence repeats (SSRs), that are one to six nucleotide repeats commonly used as molecular markers, e.g., in assessing genetic diversity. They are usually highly abundant in the genome and highly polymorphic. With advances in whole genome sequencing, the focus has also moved to mining SSRs in whole genomes (nSSRs), plastomes (cpSSRs) or mitogenomes (mtSSRs), to understand their distribution and potential function. We used assemblies originating from genome-skimming data, target-capture and publicly available sequences to survey the microsatellite content in the plastomes and nuclear gene sets of Ophioglossaceae genera. We catalogued the perfect and compound repeats, categorized them according to motif length and number of repeats and assessed microsatellite density. We examined their putative function based on the gene ontology of the groups of genes including at least one SSR. We describe both unique and shared microsatellites and discuss these findings in the light of phylogenetic relationships and other characteristics.

1 - University of Neuchatel, Laboratory of evolutionary genetics, Emile-Argand 11, Neuchatel, Neuchatel, 2000, Switzerland


Presentation Type: Colloquium Presentations
Number: C4008
Abstract ID:521
Candidate for Awards:Edgar T. Wherry award

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