Abstract Detail



Phytochemical

Uckele, Kathryn [1], Dyer, Lee [1], Philbin, Casey [1], Parchman, Thomas [1].

Landscape genomic and phytochemical variation across three juniper species and their hybrids.

Hybrid zones offer natural laboratories for understanding how genome variation underlies reproductive isolation and ecologically relevant phenotypes. Those involving foundational plant species may also allow insight into the extended consequences of admixture for higher trophic levels. Juniperus are foundational tree species in many arid regions of western North America, where they are prone to hybridization. Juniperus osteosperma (Utah juniper), J. occidentalis (western juniper), and J. grandis (Sierra juniper) come into secondary contact and hybridize in northwestern Nevada, where past work using cpDNA and nrDNA has suggested a gradient of introgression. We used a reduced-representation sequencing approach (ddRAD) to generate a data set of 8,882 SNPs spanning 326 individuals and 25 populations to infer spatial genetic structure and ancestry across pure and hybrid populations spanning Oregon, California, Nevada, and Utah. Hybrids exhibit ancestry from all three parental species, and are a mix of F1, backcrosses, and advanced Fn hybrids. Our results reveal complex patterns of ancestry in hybrid populations consistent with secondary contact and introgression. To elucidate patterns of phytochemical variation across the hybrid zone, we used untargeted gas chromatography-mass spectroscopy (GC-MS) to chemically profile our genotyped individuals, yielding relative concentrations of 163 unique chemical features. Principal components analysis of untargeted GC-MS data suggest hybrids occupy a distinct position in chemospace, which is not intermediate among parental individuals. Our ongoing analyses are examining the extent to which environmental and genetic variation jointly predict phytochemical variation across the hybrid zone, and the potential roles of transgressive variation and mismatched dominance in shaping the phytochemistry of hybrids.


1 - University Of Nevada Reno, Biology, 1664 North Virginia Street, Reno, NV, 89557, United States

Keywords:
hybrid zone
hybridization
GC-MS
ddRADseq
landscape.

Presentation Type: Oral Paper
Number: 0008
Abstract ID:41
Candidate for Awards:None


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