Abstract Detail



Systematics

Lipe, Kierstin [1], Valencia, Janice  [2], Whitten, William [3], Neubig, Kurt [4].

A phylogeny of the Orchidaceae using genome skimmed nuclear ribosomal cistrons.

Since the days of Darwin, the study of orchids has revealed an incredibly diverse array of species with dynamic evolution in need of a phylogenetic framework. Orchidaceae have been the subject of numerous molecular phylogenetic studies, the results of which are well established relationships, but based mainly in selected plastid regions. We sought to resolve a phylogenetic hypothesis of orchids using the nuclear ribosomal cistron, which has not been explored in its entirety. We genome skimmed the cistron from unenriched DNA extracts sequenced on Illumina HiSeqX with 150 bp, paired-end reads spanning 77 taxon across Orchidaceae. We analyzed the 18S, 5.8S and 26S regions for phylogenetic utility, with some degree of resolution in topology in regards to previous studies. These data show great potential as a nuclear reference for comparative genomics with plastid and mitochondrial sequences, which will be the focus of future studies.


1 - Southern Illinois University, Department of Plant Biology, Carbondale, Il, 62901-6509, USA
2 - Southern Illinois University , Plant Biology Department, 1125 Lincoln Dr., Carbondale, IL, 62901, United States
3 - Florida Museum Of Natural History, Po Box 117800, 1659 Museum Road, Gainesville, FL, 32611, United States
4 - Southern Illinois University, Dept Of Plant Biology, 1125 Lincoln Dr., Life Science II, Room 420, Carbondale, IL, 62901, United States

Keywords:
genome skimming
Nuclear ribosomal cistron
phylogeny
Orchidaceae.

Presentation Type: Poster
Session: P, Systematics
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM
Number: PSY007
Abstract ID:841
Candidate for Awards:None


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