Abstract Detail



Physiology

Ogbimi, Ejeoghene [1], Sakpere, Ayobola [2].

Shoot Regeneration and Callus Induction Potential of Node and Cotyledonary node Explants from In vitro Grown Seedlings of Afzelia  africana – An Important yet Threatened Legume.

Afzelia Africana is an important timber and food tree belonging to the family of Fabaceae and sub family Caesalpinaceae.  The in vitro shoot regeneration potential of this species was tested using nodal and cotyledonary node explants from in vitro grown seedlings.  Multiple shoots along with callus was induced from both explants – they both had potential for shoot regeneration and callus induction.  However, a better response resulted from cotyledonary node explants cultured on MS media supplemented with 15mg/L BA (Benzyladenine) and 15mg/L PG (Phloroglucinol) producing a maximum of 10 shoot buds in an explant, while nodal explants responded best on MS media supplemented with 20mg/L BA producing 3 shoots.  This study is the first report on the in vitro shoot regeneration from Afzelia africana.


1 - Department Of Botany, Obafemi Awolowo University, Ile Ife, OS, 23436, Nigeria
2 - Obafemi Awolowo University, Botany, Ile Ife, Osun, 23436

Keywords:
Node
Cotyledonary node
Benzyladenine
Phloroglucinol
Potential
Multiple shoots
Afzelia africana.

Presentation Type: Oral Paper
Number:
Abstract ID:82
Candidate for Awards:None


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