Abstract Detail

Population Genetics/Genomics

Gabbitas, Bob [1], Ickert-Bond, Steffi [2], Burleigh, J Gordon [3], Takebayashi, Naoki [4].

Phylogenetic and population genomic analyses of the Claytonia arctica complex, based on targeted sequence capture.

Morphologically variable species that inhabit a broad geographic range are a confounding issue for morphology-based classification, and risk over-splitting based on observed morphological differences. Within the Beringian flora, a group of species in the spring beauty genus (Claytonia, Montiaceae) are widespread across Beringia and morphologically highly variable, with geographically isolated populations in both western and eastern Beringia. We were interested in investigating the genetic differentiation in and around the Claytonia arctica complex sec. Panarctic Flora. We sequenced DNA from 107 individuals including 12 outgroup taxa, Claytonia arctica (9 individuals), Claytonia scammaniana (81 individuals), and Claytonia sarmentosa (5 individuals) using target-enrichment with the GoFlag angiosperm 408 probe set. The combined alignment length of the recovered loci was 365,163 nucleotides, of which 61,163 were parsimony-informative. From these sequences, we extracted SNP data, and pruned down to 1932 loci for population genetic analyses. Standard PCA and phylogenetic reconstruction indicates that C. sarmentosa is closely related to C. arctica, and probably should be considered as a member of C. arctica complex. Based on STRUCTURE analysis, we did not find any evidence of strong introgression between the three recognized species C. scammaniana, C. arctica and C. sarmentosa, suggesting that they appear to be mostly well defined taxa. However, we found a few cases of possible introgression between C. arctica and C. sarmentosa based on phylogenetic analysis in ASTRAL and STRUCTURE analysis. STRUCTURE analysis detected some level of geographic differentiation within C. scammaniana; roughly corresponding to a north-south latitudinal gradient. Two previously noted morphotypes of C. scammaniana were not differentiated from other C. scammaniana individuals in our STRUCTURE, PCA, and phylogenetic analyses. These findings change our understanding of the nature of relationships within this clade across Beringia, as well as inform expectations for how genetic diversity may be affected as warming temperatures change effective species distribution ranges within the Arctic.

1 - University of Alaska Fairbanks, Dept. of Biology & Wildlife, Fairbanks, AK, 99775, United States
2 - University Of Alaska Fairbanks, Herbarium (ALA) And Dept. Of Biology And Wildlife, University Of Alaska Fairbanks, 1962 Yukon Dr., Fairbanks, AK, 99775, United States
3 - University Of Florida, Department Of Biology, P.O. Box 118526, Gainesville, FL, 32611, United States
4 - University Of Alaska Fairbanks, Institute Of Arctic Biology, 2140 Koyukuk Dr., 311 Irving I Bldg, IAB, Fairbanks, AK, 99775, United States

Claytonia arctica complex
population genetics
GoFlag 408.

Presentation Type: Poster
Number: PPG008
Abstract ID:764
Candidate for Awards:None

Copyright © 2000-2022, Botanical Society of America. All rights reserved