Abstract Detail



Revolutionizing systematics: Herbaria in the Genomics Age

Kates, Heather [1], Folk, Ryan [2], Conde, Daniel [3], Ruben, Bella [4], Dervinis, Chris [5], LaFrance, Rafe [6], Kirst, Matias [5], Guralnick, Rob [6], Soltis, Douglas [7], SOLTIS, PAMELA S. [8].

Rapid workflows from specimens to sequences: Global-scale phylogenomics from collections.

Modern massively parallel sequencing strategies can facilitate the construction of large and robust phylogenies. Such large phylogeny projects require thousands of samples, which often present a bottleneck: relying solely on field collections may not be possible or appropriate to answer questions that require broad phylogenetic diversity. Herbaria are sources of almost limitless material for large-scale biodiversity studies, but collections usually remain untapped because of limitations in tissue sampling efficiency and DNA quality. We present a multi-part workflow to efficiently sample herbarium material and extract high yield DNA samples from tens of thousands of herbarium specimens. Our rapid tissue sampling protocol automatically captures in a linked database metadata from every specimen, including photographs. Our high throughput DNA extraction protocol is specifically suited to herbarium tissue and extracted DNA is suitable yield and quality for genomic library building and targeted enrichment sequencing. Combined, these accessible strategies dramatically decrease the time required to generate large phylogenetic trees based on phylogenomic sequence data from herbarium collections.


1 - University of Florida, PO Box 117800, Gainesville, FL, 32611-7800, United States
2 - Florida Museum Of Natural History, Dickinson Hall, 1659 Museum Road, Gainesville, FL, 32611, United States
3 - University of Florida, 2033 Mowry Road, Room 320, Gainesville, FL, 32611
4 - University of Florida, Florida Museum of Natural History, PO Box 117800, Gainesville, FL, 32611
5 - University of Florida, School of Forest Resources and Conservation, 2033 Mowry Road, Room 320, Gainesville, FL, 32611
6 - University Of Florida, Florida Museum Of Natural History, Po Box 117800, Gainesville, FL, 32611, United States
7 - Florida Museum of Natural History, University of Florida, 1659 Museum Road, Gainesville, Florida, 32611, United States
8 - Florida Museum of Natural History, University of Florida, 1659 Museum Road, Gainesville, Florida, 32611, United States

Keywords:
herbarium specimens
target enrichment
Phylogenomics
biodiversity
high throughput methods.

Presentation Type: Colloquium Presentations
Number:
Abstract ID:708
Candidate for Awards:None


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