Abstract Detail

Crops and Wild Relatives

Blain, Natalie [1], Evensen, Arthur [2].

Impact of locular gel on the formation of callus in tomato tissue cultures.

Locular gel encapsulates seeds in developing fruit. It is composed of water, sugars, carotenoids, vitamin C, lectins, and growth regulating hormones such as Abscisic acid (ABA) and gibberellins. In mature seeds, cell expansion causes the locule capsule to burst and release the locular gel. Previous studies have shown locular gel to inhibit seed germination. We hypothesized that the presence of locular gel on tomato explants would have an inhibitory effect on callus formation. In our study, two tomato fruits were sterilized and segmented into sixty explants that were cultured on Murashige and Skoog medium (MS media) with 1-Naphthaleneacetic acid (NAA) and kinetin. Thirty explants were taken from areas in direct contact with locular gel in the tomato fruit and were cultured with locular gel residue intact. The remaining thirty explants served as a control, having no locular gel contact. All explants were incubated and evaluated biweekly for callus formation. After the eighth week of the incubation period, we found no difference in callus formation between explants with locular gel contact and the control group. Our findings led us to conclude that the presence of locular gel has no discernable impact on callus formation in tomato fruit tissue culture.

1 - 445 E 500 S Apt. B8, American Fork, UT, 84003, United States
2 - 37 east 600 south, Orem, UT, 84058, USA

Tissue Culture
Locular Gel
Callus .

Presentation Type: Poster
Number: PCW002
Abstract ID:67
Candidate for Awards:None

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