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Abstract Detail

Applications of Plant Genomics

Handy, Sara [1], Zhang, Ning [1], Ramachandran, Padmini [1], Wen, Jun [2], Ottesen, Andrea [3], Timme, Ruth [3].

Development of a Reference Standard Library of Chloroplast Genome Sequences, GenomeTrakrCP.

In 2015, the total sales of herbal dietary supplements in the United Stated reached $6.92 billion, a 7.5% increase from the year before, and demand for botanicals has increased for 12 consecutive years. Many different chemical techniques have been used to monitor authenticity of supplements and more recently DNA-based tools have been included. Because questions have been raised about traditional DNA barcoding especially for processed products and closely related species, developing methods targeting smaller diagnostic regions and reference libraries for rapid species identification of plants in foods and dietary supplements would be useful and complementary to chemical methods. In the past, the United States Food and Drug Administration (FDA) has been able to develop species-specific assays targeting plant species of interest by utilizing chloroplast genome sequences. Presented here are the details for FDA’s whole chloroplast genome sequencing effort and database, known as GenomeTrakrCP. Targeted species include plants found in foods and dietary supplements as well as plants known as toxin producers. Additionally, contaminants or adulterants and closely related species to these targeted species were sequenced. All data are publically available through a bioproject in GenBank, e.g., PRJNA325670. These data are all derived from authenticated specimens and are fully annotated. Currently there are 60 complete chloroplast genomes in the database from authenticated specimens. These data can be used by FDA and other government agencies, industry and any other researcher as complete chloroplast genomes or to design species specific assays to target plant species of interest.

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1 - US Food and Drug Administration, 5001 Campus Drive, College Park, MD, 20740, USA
2 - Botany, MRC-166 National Museum Of Natural History, 10th St. & Constitution Ave., NW, Mrc 166, Washington/DC, 20013, United States
3 - US Food and Drug Administration, 5001 Campus Dr. , College Park, MD, 20740, USA

DNA-based methods

Presentation Type: Colloquium Presentations
Session: C13, Applications of Plant Genomics
Location: 104/Mayo Civic Center
Date: Wednesday, July 25th, 2018
Time: 1:45 PM
Number: C13001
Abstract ID:659
Candidate for Awards:None

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