Abstract Detail



Ethnobotany

Adongbede, Erute [1], Aduralere, Israel [2].

The use of CyQuant Cell Proliferation Assay for Screening of Compounds Extracted from Selected Macrofungi in Nigeria for Anti-Cancer Activity with Saccharomyces cerevisiae as a Model Organism.

The cell proliferation activities of polyphenols, high and low molecular weight polysaccharides extracted from macro-fungi collected from the wild in Nigeria was investigated using Saccharomyces cerevisiae as a model organism. The cell proliferation assay was carried out in a 96 well plate using the CyQuant cell proliferation assay kit. Yeast cell isolates with the different extracts from the 10 macro-fungi used (and control with no extracts) were grown in micro-plates overnight and incubated at 370C. The cells were harvested and stained with the CyQuant GR dye and the florescence read using a fluorescence micro-plate reader with excitation at ~480 nm and emission at ~520 nm. The survival rate of the model organism varied with the group of compounds and the macro-fungi. The high molecular weight polysaccharides reduced cell proliferation and the survival rate varied from 86.41% in Microporus xanthopus to 23.02% in Lentinus squarrosulus. The low molecular weight polysaccharides also varied in their effects on the model organism and survival rates varied from 103.36% in Ganoderma multipileum to 64.18% in Neonothopanus nambi. The polyphenol showed the highest anti-cell proliferation activity. Survival rates with the polyphenols varied from 59.30% in Microporus xanthopus to 21.70% in Ganoderma multipileum. The present results provide new insights into the possible therapeutic uses of mushrooms for the design of anti-tumour drugs used in combating cancer. The current research justifies the need for more research to be carried out on these groups of compounds from mushrooms as they can serve as a reservoir for drug discovery.


1 - University Of Lagos,, Department Of Botany,, Akoka, Yaba, Lagos, LA, 23401, Nigeria
2 - University of Lagos, Botany, University Road, Akoka, Yaba, Lagos, LA, 23401, Nigeria

Keywords:
Cell proliferation
Macrofungi
Polyphenols
Polysaccharide
Saccharomyces cerevisiae.

Presentation Type: Poster
Session: P, Ethnobotany
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM
Number: PET002
Abstract ID:549
Candidate for Awards:Economic Botany Section best poster


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