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Abstract Detail



Crops and Wild Relatives

Brock, Jordan [1], Beilstein, Mark [2], Olsen, Kenneth [1].

Phylogenetics of gold-of-pleasure (Camelina sativa L.) and its wild relatives.

Gold-of-pleasure (Camelina sativa) was once widely used in Europe as an oilseed crop, and has been cultivated for thousands of years for vegetable oil, lamp fuel, and animal feed. During the past 100 years, cultivation of this crop fell out of favor, resulting in the loss of cultivars and genetic diversity. The newfound application of C. sativa oil as an aviation biofuel has spurred interest in the crop once again. However, little is known about the origin of this allohexaploid species or its relationship to its diploid and polyploid congeners. We collected and analyzed wild Camelina germplasm from the region of highest diversity in Turkey and the Caucasus. These collections represent the largest and most diverse collection of Camelina germplasm known to-date. Using a subset of 54 accessions across five known species groups, we performed genome size, phylogenetic, and genetic diversity analyses to better characterize species relatedness. A double-digest restriction-site-associated DNA sequencing (ddRADseq) approach was used to generate genome-wide markers from across 44 individuals for use in downstream analyses. Results from the phylogenetic analyses show well-supported species clades. C. sativa accessions are nested within the C. microcarpa clade, suggesting that C. sativa is a domesticated form of the hexaploid species C. microcarpa. Further evidence for this inference includes similar genome size to C. microcarpa and a reduction in genetic diversity that is consistent with a domestication bottleneck. We also find one accession representing a putatively new diploid species that is sister to the tetraploid species C. rumelica, which may represent the diploid ancestor.


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1 - Washington University in St. Louis, Biology, 1 Brookings Dr., St. Louis, MO, 63130, USA
2 - University of Arizona, School of Plant Sciences, 1140 E. South Campus Drive, P.O. Box 210036, Tucson, AZ, 85721, USA

Keywords:
Camelina
domestication
genome size
RADseq
Polyploidy
Phylogenetics.

Presentation Type: Poster
Session: P, Crops and Wild Relatives
Location: Grand Ballroom - Exhibit Hall/Mayo Civic Center
Date: Monday, July 23rd, 2018
Time: 5:30 PM This poster will be presented at 6:15 pm. The Poster Session runs from 5:30 pm to 7:00 pm. Posters with odd poster numbers are presented at 5:30 pm, and posters with even poster numbers are presented at 6:15 pm.
Number: PCW008
Abstract ID:448
Candidate for Awards:None


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