Abstract Detail

Bryology and Lichenology

Shi, Shuo [1], Li, Lin [1], Shen, Fengjiao [1], Niu, Jingyuan [2], Li, Shuangling [1], Liu, Yanlei [3], Zhao, Jiancheng [1].

The Preliminary Molecular Systematic Study of Bryales (Bryophyta).

Bryales is a large order of mosses, which plays an important role in the systematic study of bryophytes. However, the subdivision classification of Bryales is still in suspense. There are three main families (in some systems), Bryaceae, Mniaceae, and Mielichhoferiaceae, corresponding to three main clades under Bryales. But the relationship and circumscription of these three families are not clear. In this study, the molecular and other data were integrative to solve this problem to some degree. We tried to develop new techniques: collect better DNA specimens in the field, preserve DNA material for a longer time in the lab, repair herbaria specimen DNA, extract trace amounts of DNA, and PCR. Based on the (deep) genome skimming of NGS, the chloroplast genome, mitochondria genome, rDNA, and dozens of nuclear genes of Bryale’s main clades were achieved. The comparison and evolutionary study of organelle genomes of Bryales were conducted, and the molecular systematic analysis and origin time was analyzed. And we developed the DNA barcodes suitable for systematic research in Bryales based on chloroplast-genome data. We used them in the systematic study of a more extensive number sample analysis. The main results and conclusions are: family Mielichhoferiaceae is nested in Mniaceae, they are the sister group of family Bryaceae in Bryales; the origin time of Bryales is Eocene, the extreme warmth of the early Eocene may have influenced the morphology characteristics of Bryales species which used for classification; genus Pohlia is not a monophyletic group, the further taxonomy and treatment are needed.

1 - Hebei Normal University, College of Life Sciences, No.20, East Nanerhuan Road, Shijiazhuang, Hebei, 050024, China
2 - Xinxiang Medical University, School of Basic Medical Sciences, 601 Jinsui Avenue, Xinxiang, Henan, 453003, China
3 - Hebei University of Engineering, School of Landscape and Ecological Engineering, No.19 Taiji Road, Economic and Technological Development Zone, Handan, Hebei, 056038, China

genome skimming
DNA barcoding

Presentation Type: Oral Paper
Number: BL1005
Abstract ID:425
Candidate for Awards:None

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