Hou, Chen , Saunders, Richard M.K. , Wikström, Niklas , Strijk, Joeri S. , Rydin, Catarina .
High-throughput sequencing of chloroplast genomes to resolve a rapidly evolving lineage - a case study in Chinese lianoid Gnetum (Gnetales).
Rapid species diversification is omnipresent in the evolutionary history of seed plants, and may hamper assessments of phylogeny and species boundaries. An example of this is found in Chinese Gnetum (Gnetales), which consist of eight liana and one arborescent species distributed in tropical/subtropical rain forests of South China. A preliminary estimate of speciation rates using a MEDUSA-like model reveals that Chinese lianoid species evolved rapidly compared with other Asian lianoid and arborescent lineages in Gnetum. Results of a recent phylogenetic study and dating analysis indicate that Chinese lianoid Gnetum originated and diverged around 23 Ma (95% highest posterior density, 8-39 Ma). Further conclusions on evolution in the clade are, however, precluded due to lack of phylogenetic resolution. To address this, and problems with species delimitations, we are generating plastid genomic data of Chinese Gnetum species using next generation sequencing (NGS) methods. To date, 11 complete chloroplast genomes that represent five of the nine species and two variants have been assembled, and sequences have been aligned to detect informative regions. Phylogenies based on protein coding/non-coding regions, and entire chloroplast genomes have been constructed separately and compared to pave the way for additional work using targeted Sanger sequencing methods and extensive field samples collected mainly in Southern China. The output data will be used for biogeographic, phylogeographic studies, and also for taxonomic work and conservation biology.
1 - Stockholm University, Department of Ecology, Environment and Plant Sciences, Lilla Frescati 5, Stockholm, Stockholm, 10691, Sweden
2 - UNIVERSITY OF HONG KONG, POKFULAM ROAD, HONG KONG, N/A, Hong Kong SAR
3 - Guangxi University, Plant Ecophysiology & Evolution Group, College of Forestry, Da Xue Dong Lu 100, Nan Ning, Guang Xi, 530005, China
Presentation Type: Oral Paper:Papers for Topics
Candidate for Awards:Margaret Menzel Award