Abstract Detail

Population Genetics/Genomics

Liu, Luxian [1], Wang, Yuewen [1], He, Peizi [1], Lee, Joongku [2], Li, Pan [3], Soltis, Douglas [4], Fu, Chengxin [3].

Chloroplast genome analyses and genomic resource development for epilithic sister genera Oresitrophe and Mukdenia (Saxifragaceae), using genome skimming data.

Background: Epilithic sister genera Oresitrophe and Mukdenia (Saxifragaceae) have an epilithic habitat (rocky slopes) and a parapatric distribution in East Asia, which makes them an ideal model for a more comprehensive understanding of the demographic and divergence history and the influence of climate changes in East Asia. However, the genetic background and resources for these two genera are scarce.
Results: The complete chloroplast (cp) genomes of two Oresitrophe rupifraga and one Mukdenia rossii individuals were reconstructed and comparative analyses were conducted to examine the evolutionary pattern of chloroplast genomes in Saxifragaceae. The cp genomes ranged from 156,738 bp to 156,960 bp in length and had a typical quadripartite structure with a conserved genome arrangement. Comparative analysis revealed the intron of rpl2 has been lost in Heuchera parviflora, Tiarella polyphylla, M. rossii and O. rupifraga but presents in the reference genome of Penthorum chinense. Seven cp hotspot regions (trnH-psbA, trnR-atpA, atpI-rps2, rps2-rpoC2, petN-psbM, rps4-trnT and rpl33-rps18) were identified between Oresitrophe and Mukdenia, while four hotspots (trnQ-psbK, trnR-atpA, trnS-psbZ and rpl33-rps18) were identified within Oresitrophe. In addition, 24 polymorphic cpSSR loci were found between Oresitrophe and Mukdenia. Most importantly, we successfully developed 126 intergeneric polymorphic gSSR markers between Oresitrophe and Mukdenia, as well as 452 intrageneric ones within Oresitrophe. Twelve randomly selected intergeneric gSSRs have shown that these two genera exhibit a significant genetic structure.
Conclusions: In this study, we conducted genome skimming for Oresitrophe rupifraga and Mukdenia rossii. Using these data, we were able to not only assemble their complete chloroplast genomes, but also develop abundant genetic resources (cp hotspots, cpSSRs, polymorphic gSSRs). The genomic patterns and genetic resources presented here will contribute to further studies on population genetic, phylogeny and conservation biology in Saxifragaceae.

1 - Henan University, College of Life Sciences, 85 Minglun St., Kaifeng, Henan, 475000, China
2 - Chungnam National University, Department Of Environment & Forest Resources, 99 Daehak-ro, Yuseong-Gu, Daejeon, 30, 34134, Korea, Republic of
3 - Zhejiang University, College Of Life Sciences, 866 Yuhangtang Rd., Hangzhou, Zhejiang, 310058, China
4 - University of Florida, Biology, Gainesville, FL

Chloroplast genome
cp hotspot
East Asia
population genetics

Presentation Type: Poster
Number: PPG001
Abstract ID:350
Candidate for Awards:None

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