Abstract Detail

Hybrids and Hybridization

Uckele, Kathryn [1], Parchman, Thomas [2], Richards, Lora [2], Shelef, Oren  [2].

Landscape genomic variation across three juniper species and their hybrids.

Plant hybrid zones offer natural laboratories for understanding how genome variation underlies reproductive isolation and ecologically relevant phenotypes. By studying hybrid zones, we can  better understand environmental constraints on hybridization and the cascading effects hybridization may have on higher trophic levels. Juniperus are foundational tree species in many arid regions of western North America, where they are prone to hybridization. Juniperus osteosperma (Utah juniper), J. occidentalis (western juniper), and J. grandis (Sierra juniper) come into secondary contact and hybridize in northwestern Nevada, where past work using cpDNA and nuclear ribosomal DNA has suggested a gradient of introgression. We generated a data set of 8,882 SNPs spanning 25 populations using a reduced-representation sequencing approach (ddRAD) to generate a finer scale understanding of population genetic structure and to infer ancestry across pure and potential hybrid populations spanning Oregon, California, Nevada, and Utah. Utilizing ecological niche modeling and environmental association analysis, we evaluate the extent to which environmental variation may shape genome variation and hybrid ancestry across geographic space. Our results reveal complex patterns of ancestry in hybrid populations, with many having ancestry of all three parental species, and suggest a role for environmental variation in shaping genome variation and hybridization. We are currently employing untargeted metabolomic methods in order to evaluate how environment, genome variation, and hybridization predict phytochemical variation and its potential ecological consequences.

1 - University of Nevada Reno, Biology, 1664 N. Virginia Street, Reno, NV, 89503, USA
2 - University of Nevada Reno, 1664 N. Virginia Street, Reno, NV, 89503, United States

population genetics
landscape genetics.

Presentation Type: Oral Paper
Number: 0005
Abstract ID:305
Candidate for Awards:Margaret Menzel Award

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