Abstract Detail



Functional Genetics/Genomics

Gebken, Sarah [1], Mabry, Makenzie [2], PIRES , JOSEPH CHRIS [3].

Making a Better Kale: Implementing a Novel Genetic Engineering Technique for Brassica oleracea.

CRISPR (Clustered Regularly Interspaced Palindromic Repeats) is a genetic engineering tool that is revolutionizing the field of biology because it is a precise and inexpensive method for introducing mutations in organisms. In this project, we are editing a gene that affects leaf shape using CRISPR/Cas9 System. The gene we are attempting to knockout is CURLY LEAF (CLF), shown in Arabidopsis thaliana to display reduced stem dimensions as well as curled and reduced foliage leaves. Arabidopsis is closely related to Brassica oleracea which includes vegetable crops such as kale, cauliflower, and broccoli. However, Brassica has many more duplicated genes than Arabidopsis, making it more challenging to study and to target genes to knock-out and edit. Using two guide RNAs to target two parts of a gene, one at the beginning of the gene and one at the end, we can increase the likelihood of a complete knock-out of the gene. Our goal is to increase our understanding of the CRISPR/Cas 9 system by allowing us to study how we can overcome duplicated genes in Brassica oleracea to find an efficient transformation process with CRISPR. After a successful knockout of CLF in Brassica oleracea, we will use the same methods in Brassica napus (canola, rutabaga), which has even more duplicate genes than Brassica oleracea. Using a combination of lab work and bioinformatics, our goal is to find a general way to predictably increase genetic engineering efficiency in a variety of organisms with duplicated genes and genomes.


1 - University of Missouri - Columbia, Biological Engineering, 416 S 6th St, Columbia, MO, 65201, USA
2 - University Of Missouri, Biological Sciences, 1201 Rollins St., Columbia, MO, 65201, United States
3 - University Of Missouri, 371 Bond Life Sciences Center, 1201 Rollins Street, Columbia, MO, 65211, United States

Keywords:
CRISPR
Brassica.

Presentation Type: Poster
Number: PCG002
Abstract ID:131
Candidate for Awards:None


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